【著書（分担執筆）】
『栄養・食糧学用語辞典』日本栄養・食糧学会編、建帛社、2007年10月　栄養・食糧学用語辞典を分担執筆した。

【学術論文】
Yukiko Ishitsuka*, Fumio Maniwa*, Chiharu Koide*, Natsuko Douzaki, Yoji Kato, Yoshimasa Nakamura**, Toshihiko Osawa***
*KOSE´ Corporation、**Okayama University Graduate School of Natural Science and Technology、 ***Nagoya University Graduate School of Bioagricultural Sciences
Detection of modified tyrosines as an inflammation marker in a photo-aged skin model
Photochemistry and Photobiology, 83 (3) pp. 698-705. 2007年5月
　Reactive nitrogen species, produced during the process of inflammation induced by various factors including UV radiation, modify amino acids in crucial proteins. To investigate the influence of the modified tyrosine on UV-exposed skin, we detected the nitrotyrosine or halogenated tyrosine and dityrosine in photo-aged model mice. In this study, we used a low dose of UV irradiation to which we are exposed in daily life. Our results suggest that UV exposure in daily life may induce the production of modified tyrosines and skin aging.

兵庫県内の河川源流、上流および名水の総括的水質調査
北元憲利先生との共同研究
北元研究室Website

Aoi W*, Naito Y*, Takanami Y*, Ishii T**, Kawai Y＊, Akagiri S＊, Kato Y, Osawa T***, Yoshikawa T*.
*Kyoto Prefectual University of Medicine、**Univerisity of Shizuoka、***Nagoya University Graduate School of Bioagricultural Sciences
Astaxanthin improves muscle lipid metabolism in exercise via inhibitory effect of oxidative CPT I modification
Biochem. Biophys. Res. Commun., 366, pp. 892-897. 2008年2月
　The present study investigated the effect of astaxanthin on muscle lipid metabolism in exercise. ICR mice (8 weeks old) were divided into four different groups: sedentary, sedentary treated with astaxanthin, running exercise, and exercise treated with astaxanthin. Our results suggested that astaxanthin promoted lipid metabolism rather than glucose utilization during exercise via CPT I activation, which led to improvement of endurance and efficient reduction of adipose tissue with training.

【学会口頭及びポスター発表】
石塚由紀子*、間庭史雄*、小出千春*、堂崎奈津子、加藤陽二、中村宜督**、大澤俊彦***
*コーセー研究所、**岡大院・自然科学、***名大院・生命農学
「光老化皮膚におけるチロシン修飾物の検出」　日本フリーラジカル学会/日本過酸化脂質フリーラジカル学会合同開催　2007年6月
　紫外線は皮膚の肥厚や深いシワの形成をもたらすと考えられているがその直接的な関与についての報告は少ない。タンパク質を修飾する活性窒素種に着目し、その反応産物であるチロシン修飾物の検出を行ったところ、UV照射によりひきおこされた炎症の過程で修飾チロシンが生成された。今回のUV照射線量は日常レベルであることから、我々の結果は日常的な紫外線暴露が修飾チロシンの生成を誘導する可能性を示すものである。

志葉優子*、河合慶親*、加藤陽二、木下崇司*、中馬寛*、寺尾純二*
*徳大院・医
「ケルセチンによるミエロペルオキシダーゼ阻害機構の検討」　日本フリーラジカル学会/日本過酸化脂質フリーラジカル学会合同開催　2007年6月
　ケルセチン及びその配糖体によるミエロペルオキシダーゼ(MPO)阻害の機構についてジチロシン生成を指標に検討し、コンピューターシミュレーションによるドッキング研究も行った。

石塚由紀子*、間庭史雄*、小出千春*、堂崎奈津子、加藤陽二、中村宜督**、大澤俊彦***
*コーセー研究所、**岡大院・自然科学、***名大院・生命農学
「光老化皮膚におけるチロシン修飾物の検出」　光老化研究会　2007年6月
前掲「光老化皮膚におけるチロシン修飾物の検出」参照

中村 俊之、河合 慶親*、北元 憲利、大澤 俊彦**、加藤 陽二
*徳大院・医、**名大院・生命農学
「イソチオシアネート類とリジン残基の反応について」日本農芸化学会　2008年3月
　ワサビの辛み成分であるアリルイソチオシアナートとリジン残基の反応について中性条件下で調べたところ、蛋白質SH基とも反応するが、リジン残基にも付加修飾することが化学的及び免疫化学的な手法により明らかとなった。

日坂 真輔*、加藤 陽二、丸山 和佳子**、大澤 俊彦*
名大院・生命農学*、長寿医療センター**
「生体内脂質過酸化による酸化傷害機構の化学的解明」日本農芸化学会　2008年3月
　ω３系脂肪酸の酸化に伴う脳内蛋白質の修飾について調べたところ、タウ蛋白質がプロパノイル化されていることを免疫沈降法などにより明らかにした。

【学会誌ニュースレター】
加藤陽二「炎症反応とガン予防」　日本がん予防学会ニュースレター　2008年3月
　ガン予防のためには抗炎症をターゲットに考える必要性があることと、ニュージーランドにおける最新の関連研究を紹介した。

国際学会発表

Yoji Kato, Natsuko Dozaki, Manami Sugita, Noritoshi Kitamoto, Toshihiko Osawa*
*Nagoya University Graduate School of Bioagricultural Sciences
Inhibitory effect of phenolic antioxidants on eosinophil peroxidase
3rd International Conference of Polyphenol and Health (ICPH)　2007年11月
　In this study, two methods for evaluation of inhibitory effects of eosinophil peroxidase (EPO) were established. Phenolic antioxidants strongly inhibited the formation of modified Tyr and the inhibitory effects increased with increasing phenolic OH moieties. Tocopherol, butylated hydroxytoluene (BHT), and cinnamic acid could not inhibit. Reduced and oxidized glutathione can inhibit the bromination but not dimerization of tyrosine, suggesting glutathione inhibit HOBr-derived modification but not HOBr generation itself from EPO.

Yoji Kato, Toshiyuki Nakamura, Yoshichika Kawai*, Noritoshi Kitamoto, Toshihiko Osawa**
*Tokushima University, **Nagoya University Graduate School of Bioagricultural Sciences
Modification of lysine by isothiocyanates
International Conference of Food Factor for Health Promotion 2007 (ICoFF2007)　2007年11月
　The chemical reaction of ITC with bovine serum albumin (BSA) has been investigated. The amounts of lysine in acid hydrolysates of BSA were specifically reduced by ITC treatment. Next, Bz-Gly-Lys (Bz-GK) was reacted with ITC in buffers. New products from the reaction mixtures were detected and then identified as N-thiocarbamoyl-adducts by 1H-NMR and mass spectrometry. The adduct formation between Bz-Gly-Lys and grated Sawa-Wasabi was examined using LC/MS/MS. Both Allyl-ITC and 6-Methlsulfinylhexyl-adducts were detected in the mixture and the formation was increased with increasing amounts of Wasabi.

Toshiyuki Nakamura, Noritoshi Kitamoto, Toshihiko Osawa*, Yoji Kato
*Nagoya University Graduate School of Bioagricultural Sciences
Preparation and characterization of a monoclonal antibody specific to allyl　isothiocyanate-lysine adducts in proteins
International Conference of Food Factor for Health Promotion 2007 (ICoFF2007)　2007年11月
　When bovine serum albumin (BSA) was incubated with allyl isothiocyanate (AITC), considerable loss of Lys residues was observed. The antibody to AITC-protein was prepared and characterized. Using the antibody, we detected AITC-Lys from cultured cell by immunocytochemical staining. The formation of AITC-Lys in vivo following intake of Wasabi AITC is to be assessed.

Shinsuke Hisaka*, Yoji Kato, Noritoshi Kitamoto, Yoshiko Kubushiro**, Michitaka Naito**, Wakako Maruyama***, Toshihiko Osawa*
*Nagoya University Graduate School of Bioagricultural Sciences, **Sugiyama Jogakuen University, ***National Institute for Longevity Sciences
Immunohistochemical detection of N-epsilon-propanoyl-lysine produced by reaction of lysine with oxidized omega-3 polyunsaturated fatty acids
International Conference of Food Factor for Health Promotion 2007 (ICoFF2007)　2007年11月
　To evaluate DHA or EPA-derived protein modification in vivo, we prepared and characterized a novel monoclonal antibody against propanoyl-lysine, PRL. The strong positive staining of atherosclerotic lesions from hypercholesterolemic rabbits was observed. In addition, specimens of old rat brain were more strongly stained by the antibody than these of young brain, suggesting enhancement of oxidative stress and/or failure of clearances of oxidatively modified biomolecules during brain aging.

Yoji Kato, Tim Harwood*, Alexander Peskin*, Christine Winterbourn*, Anthony Kettle*
*Free Radical Research Group, Department of Pathology, University of Otago
Covalent modification of protein thiols by serotonin oxidation products formed by the catalytic action of myeloperoxidase
The 4th Joint Meeting of the Society for Free Radical Research Australasia and Japan (SFRR A+J)　2007年12月
　We examined the modification of thiols by serotonin in the presence of myeloperoxidase (MPO) and xanthine oxidase. In the presence of N-acetylcysteine (NAC), serotonin-dimer and three major NAC adducts were formed. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a model thiol protein. Preliminary liquid chromatography mass spectrometry-based proteomic analysis indicated that one of adducts formed when TD reacted with the protein involved the peptide containing Cys-244. We propose that during inflammation MPO will oxidize serotonin resulting in covalent modification of thiol residues on proteins.

Tomohisa Takagi*, Yuji Naito*, Hitomi Okada*, Yayoi Nukigi*, Kazuhiko Uchiyama*, Masaaki Kuroda*, Nobuaki Yagi*, Naoya Tomatsuri*, Osamj Handa*, Satoshi Kokura*, Yoji Kato, Yoshihiko Osawa**, Toshikazu Yoshikawa*
*Kyoto Prefectual University of Medicine、**Nagoya University Graduate School of Bioagricultural Sciences
The expression of nitric oxide (NO)-modified proteins in Helicobacter Pylori-associated inflamed gastric mucosa
The 4th Joint Meeting of the Society for Free Radical Research Australasia and Japan (SFRR A+J)　2007年12月
　The data shown that iNOS mRNA expression and NO-modified protein in H.pylori-associated atrophic gastric mucosa were increased. These modified proteins might be involved in gastric carcinogenesis.

Yoji Kato, Shinsuke Hisaka*, Akihiro Yoshida**, Yoshiko Fukuchi***, Michitaka Naito***, Wakako Maruyama****, Noritoshi Kitamoto, Toshihiko Osawa*
*Nagoya University Graduate School of Bioagricultural Sciences、**Nakatsugawa Municipal Hospital、***Sugiyama Jogakuen University, **** National Institute for Longevity Sciences
Novel biomarker of oxidative stress: oxidized omega-3 fatty acid-lysine adducts
International Symposium on Biomarkers of Oxidative Stress in Health and Diseases (BOSHD2008) 　2008年1月
　In this study, we have reported that propanoyl-lysine (propionyl-lysine, PRL), as an oxidized omega-3 fatty acid-derived lysine adduct, was quantified from human urine by LC/MS/MS with isotopic dilution method. The amounts of urinary PRL and hexanoyl-lysine (HEL) in diabetic urines were higher than those of control human urines. Using the specific anti-PRL antibody, the presence in atherosclerotic plaque of cholesterol-fed rabbit has been confirmed. These lipid-lysine amide-type adducts family may be useful biomarkers for oxidative stress in vivo.